Measurement of point-spread function (PSF) for confocal fluorescence microscopy

InCheon Song; HongKi Yoo; Jaebum Choo; Dae-Gab Gweon

doi:10.1117/12.616701

31 August 2005 Measurement of point-spread function (PSF) for confocal fluorescence microscopy

InCheon Song, HongKi Yoo, Jaebum Choo, Dae-Gab Gweon

Proceedings Volume 5878, Advanced Characterization Techniques for Optics, Semiconductors, and Nanotechnologies II; 58781B (2005) https://doi.org/10.1117/12.616701
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

In this paper, we describe size-effect of fluorescent microsphere for measuring point-spread function (PSF) in confocal fluorescence microscopy. We present the numerical results for the practically available microsphere size range for measuring PSF, and demonstrate with experiment. Also, the effective PSF is restored with deconvolution technique within an acceptable error. Also the size-effect for measuring phase modulated PSF, which has two vicinal peaks, is described. The numerical and experimental result is also presented.

Citation Download Citation

InCheon Song, HongKi Yoo, Jaebum Choo, and Dae-Gab Gweon "Measurement of point-spread function (PSF) for confocal fluorescence microscopy", Proc. SPIE 5878, Advanced Characterization Techniques for Optics, Semiconductors, and Nanotechnologies II, 58781B (31 August 2005); https://doi.org/10.1117/12.616701

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