Observing the dynamic variation of the binding force between rhodostomin ligand and integrin alpha(IIb)beta(3) receptor using photonic force microscope

Bo-Jui Chang; Chia-Fen Hsieh; Chi-Hung Lin; Sien Chi; Long Hsu

doi:10.1117/12.559501

18 October 2004 Observing the dynamic variation of the binding force between rhodostomin ligand and integrin α_IIbβ₃ receptor using photonic force microscope

Bo-Jui Chang, Chia-Fen Hsieh, Chi-Hung Lin, Sien Chi, Long Hsu

Author Affiliations +

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.559501
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

The conformational change of integrin α_IIbβ₃ plays an important role in clot formation. However, the correlation between the structure and the function of integrin α_IIbβ₃ in interacting with its ligand is still not clear. In this report, we focus on the dynamic variation of the binding between integrin α_IIbβ₃ and its ligand, rhodostomin by using a photonic force microscopy (PFM). The PFM is used to trap a rhodostomin-coated bead and, then, shift it to bind a surrounding CHO α_IIbβ₃ cell. Meanwhile, it tracks, with a resolution of 1MHz, the Brownian fluctuations of the trapped bead. Theoretically, the smaller the amplitude of the Brownian fluctuations, the stronger the stiffness of the binding force between the rhodostomin and the CHO α_IIbβ₃ cell. Experimentally, a significant decrease of the Brownian fluctuations was observed during the interval between the 360th seconds and the 400th seconds after the trapped rhodostomin-coated bead contacted an integrin-expressed CHO α_IIbβ₃ cell. This observation reveals that it takes the rhodostomin 360 seconds to seek the correct position to bind to the integrin α_IIbβ₃. After 400 seconds, the rhodostomin has bound rigidly with the integrin α_IIbβ₃. We presume that the integrin α_IIbβ₃ has reached its final stage of conformational change.

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Bo-Jui Chang, Chia-Fen Hsieh, Chi-Hung Lin, Sien Chi, and Long Hsu "Observing the dynamic variation of the binding force between rhodostomin ligand and integrin α_IIbβ₃ receptor using photonic force microscope", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.559501

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KEYWORDS

Scattering

Laser scattering

Light scattering

Microscopes

Optical tweezers

Signal detection

Photonic microstructures

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