Monitoring of DsRed protein concentration in frozen insect larvae

Yordan Kostov; Leah Tolosa; Kevin O'Connell; Patricia Anderson; Yi Liu; Nikolai van Beek; Govind Rao

doi:10.1117/12.485720

12 September 2003 Monitoring of DsRed protein concentration in frozen insect larvae

Yordan Kostov, Leah Tolosa, Kevin O'Connell, Patricia Anderson, Yi Liu, Nikolai van Beek, Govind Rao

Author Affiliations +

Proceedings Volume 4967, Genetically Engineered and Optical Probes for Biomedical Applications; (2003) https://doi.org/10.1117/12.485720
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

A baculovirus expression system was used to produce DsRed fusion protein in insect larvae. As the baculovirus/insect larvae system requires precise harvest timing to achieve high yield of protein, a low-cost miniature all-solid state optical probe was used for detection of the protein concentrations in the frozen larvae. Three batches of infected larvae were monitored at different post-infection times. The calibration curve of the probe was obtained by simultaneous measurements both in laboratory fluorimeter and using gel electrophoresis analysis. The results show good correlation between the optical measurements and the standard laboratory technique.

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Yordan Kostov, Leah Tolosa, Kevin O'Connell, Patricia Anderson, Yi Liu, Nikolai van Beek, and Govind Rao "Monitoring of DsRed protein concentration in frozen insect larvae", Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); https://doi.org/10.1117/12.485720

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