Gene expression analysis of perennial ryegrass (Lolium perenne) using cDNA microarrays

Eng-Kok Ong; Tim Sawbridge; Tracie Webster; Michael Emmerling; Nga Nguyen; Katrina Nunan; Matthew O'Neill; Fiona O'Toole; Carolyn Rhodes; Jason Simmonds; Pei Tian; Katherine Wearne; Amanda Winkworth; German Spangenberg

doi:10.1117/12.478075

18 July 2003 Gene expression analysis of perennial ryegrass (Lolium perenne) using cDNA microarrays

Eng-Kok Ong, Tim Sawbridge, Tracie Webster, Michael Emmerling, Nga Nguyen, Katrina Nunan, Matthew O'Neill, Fiona O'Toole, Carolyn Rhodes, Jason Simmonds, Pei Tian, Katherine Wearne, Amanda Winkworth, German Spangenberg

Author Affiliations +

Proceedings Volume 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis; (2003) https://doi.org/10.1117/12.478075
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Perennial ryegrass (Lolium perenne) is a major forage grass of temperate pastures. A genomics program has been undertaken generating over 52,000 expressed sequence tags (ESTs). Cluster analysis of the ESTs identified approximately 14,600 ryegrass unigenes. In this report, we described the application of ryegrass unigene cDNAs to produce ryegrass 15K microarray. Fifteen microarray hybridisations were performed with labeled total RNA isolated from a variety of plant organs and developmental stages. In a proof of concept, gene expression profiling of ryegrass ESTs using the 15K unigene microarrays has been established using several known genes and two cluster analysis approaches (parallel coordinate planes plot and hierarchical clustering). The expression profile of the known genes (e.g. rubisco and invertase) corresponds well with published data. The microarray expression profile of a ryegrass putative root specific kinase gene was also verified with Northern blotting. This combination of DNA microarray hybridisations and cluster analysis can be applied as a tool for the identification of novel sequences of unknown function.

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Eng-Kok Ong, Tim Sawbridge, Tracie Webster, Michael Emmerling, Nga Nguyen, Katrina Nunan, Matthew O'Neill, Fiona O'Toole, Carolyn Rhodes, Jason Simmonds, Pei Tian, Katherine Wearne, Amanda Winkworth, and German Spangenberg "Gene expression analysis of perennial ryegrass (Lolium perenne) using cDNA microarrays", Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); https://doi.org/10.1117/12.478075

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