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3 July 2003 Polarized light (λ400-2000nm): a description of the wound healing process using immunohistochemical analysis
Bruno Pereira Campanha, Antonio Luiz Barbosa Pinheiro, Ruben Weismann, Marilia Gerhardt de Oliveira, Luciana Maria Pedreira Ramalho
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Proceedings Volume 4950, Lasers in Dentistry IX; (2003) https://doi.org/10.1117/12.476430
Event: Biomedical Optics, 2003, San Jose, CA, United States
Abstract
Wound healing is a complex process that involves several metabolic and cellular responses. Several methods of improving wound healing have been suggested. Polarized light was suggested as a new method of biostimulation, improving the quality of the healing process. The aim of this work was to investigate the effects of polarized light (λ400 - λ2000nm) on the healing of cutaneous wounds using immunohistochemical analysis. Eighteen healthy male young adults Wistar rats were used. Standardized wounds were created on the dorsum of the rat. The wounds were illuminated with the polarized light source at doses of 20 or 40J/cm2. Non-illuminated animals acted as controls. The first application of the light was performed immediately after the surgical procedure and repeated at every 48 hours during seven days. Twenty-four hours after the last irradiation the animals were humanely sacrificed. The slices were immunomarked by α SMA and by Picrosírius stain. The results of the histological analysis shows that the healing process on illuminated subjects was characterized by increased amount of collagen fibers when compared to control wounds. On the other hand, expressive amounts of myofibroblasts were also observed on illuminated specimens, when compared to non-irradiated animals, especially with a dose of 20J/cm2. It is concluded that the use of polarized light had influence on the proliferation of myofibroblasts as well as on the amount of collagen fibers, being this best observed when small doses are used.
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Bruno Pereira Campanha, Antonio Luiz Barbosa Pinheiro, Ruben Weismann, Marilia Gerhardt de Oliveira, and Luciana Maria Pedreira Ramalho "Polarized light (λ400-2000nm): a description of the wound healing process using immunohistochemical analysis", Proc. SPIE 4950, Lasers in Dentistry IX, (3 July 2003); https://doi.org/10.1117/12.476430
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KEYWORDS
Wound healing
Collagen
Control systems
Laser dentistry
Light sources
Shape memory alloys
Microscopy
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