Simultaneous measurement of photosensitizer absorption and fluorescence in patients undergoing photodynamic therapy

Alexander A. Stratonnikov; Natalia V. Ermishova; Gennadii A. Meerovich; Boris V. Kudashev M.D.; Elena G. Vakoulovskaya M.D.; Victor B. Loschenov

doi:10.1117/12.465242

1 May 2002 Simultaneous measurement of photosensitizer absorption and fluorescence in patients undergoing photodynamic therapy

Alexander A. Stratonnikov, Natalia V. Ermishova, Gennadii A. Meerovich, Boris V. Kudashev M.D., Elena G. Vakoulovskaya M.D., Victor B. Loschenov

Author Affiliations +

Proceedings Volume 4613, Optical Biopsy IV; (2002) https://doi.org/10.1117/12.465242
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

This paper deals with photosensitizer quantification in patients undergoing photodynamic therapy. Both fluorescence and diffuse reflectance spectroscopy were applied to evaluate concentration of sulphonated aluminum phthalocyanine in different tissues. The mixtures of Intralipid, blood and photosensitizer with different concentrations were used as standard samples to solve the problem in question. While fluorescence method is more sensitive and more convenient to apply in clinics, the absorption technique may be applied to non fluorescent dyes and used to evaluate the shifts in adsorption peak position due to interaction of dye with tissues. Finally, the concentration dynamics of non fluorescent dye (cobalt phthalocyanine) in patients was obtained with the use of absorption method alone.

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Alexander A. Stratonnikov, Natalia V. Ermishova, Gennadii A. Meerovich, Boris V. Kudashev M.D., Elena G. Vakoulovskaya M.D., and Victor B. Loschenov "Simultaneous measurement of photosensitizer absorption and fluorescence in patients undergoing photodynamic therapy", Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); https://doi.org/10.1117/12.465242

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