Action of the photosensitizer QLT0074 upon human T lymphocytes in vitro

David W. C. Hunt; Huijun Jiang; Ruth A. Salmon; David J. Granville; John R. North; Anna M. Richter

doi:10.1117/12.424443

9 April 2001 Action of the photosensitizer QLT0074 upon human T lymphocytes in vitro

David W. C. Hunt, Huijun Jiang, Ruth A. Salmon, David J. Granville, John R. North, Anna M. Richter

Proceedings Volume 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X; (2001) https://doi.org/10.1117/12.424443
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

A new photosensitizer, presently designated QLT0074, may be useful for the treatment of skin conditions, particularly those mediated by T lymphocytes, with photodynamic therapy (PDT). QLT0074 was tested against human peripheral blood T cells and Jurkat T lymphoma cells. Low concentrations of QLT0074 and blue light were sufficient to induce apoptosis in peripheral blood T cells or Jurkat T lymphoma cells as indicated by expression of the apoptosis-associated mitochondrial 7A6 marker, annexin-V labeling or activation of capsase-3 and cleavage of the capsase substrate poly(ADP-ribose)polymerase (PARP). Flow cytometry studies performed following PDT showed that peripheral blood T cells with high expression of the interleukin-2 receptor (CD25) took up greater amounts of QLT0074 and were eliminated to a greater degree than T cells with low CD25 levels. This effect of PDT was also shown by the reduction in the percentage of T cells that expressed other activation-associated markers such as very late activation antigen-4 (CD49d), human leukocyte antigen DR (HLA-DR), intercellular adhesion molecule-1 (CD54) and Fas (CD95). In the case of T cells that remained viable following PDT, CD25 expression was lower while CD54, CD95 and HLA-DR levels were unchanged. Thus, PDT with QLT0074 has selective, dose-dependent effects on T cells in vitro.

Citation Download Citation

David W. C. Hunt, Huijun Jiang, Ruth A. Salmon, David J. Granville, John R. North, and Anna M. Richter "Action of the photosensitizer QLT0074 upon human T lymphocytes in vitro", Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); https://doi.org/10.1117/12.424443

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KEYWORDS

Photodynamic therapy

Cell death

Blood

Luminescence

Monoclonal antibodies

Receptors

In vitro testing

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