Application of phase contrast microscopy to the study of marine microbiota

MingChang Shih; Chang Ten Lian; J. S. Hwang

doi:10.1117/12.390552

4 July 2000 Application of phase contrast microscopy to the study of marine microbiota

MingChang Shih, Chang Ten Lian, J. S. Hwang

Proceedings Volume 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications; (2000) https://doi.org/10.1117/12.390552
Event: Photonics Taiwan, 2000, Taipei, Taiwan

Abstract

We present the observation of the activity of artemia, one of the popular marine micro-biota species, in free space by the application of Fourier optics imaging technique. The Fourier optic imaging system is consisted by a collimated laser beam source, a Fourier spatial filter, an non-coherent IR source, and a CCD imaging system. By recording the images of Artemia's motion in real life, we are able to study the fundamental patterns of artemia motion mechanism, and the response of the motion pattern to the variation of its environment. Characteristic patterns of artemia's motion, such as linear motion, spiral motion, and mating collision are observed. It is shown that the increasing of the environment temperature driving the motion of the artemia's moving faster and more frequently, and still stays alive even at the environment temperature up to 38 C.

Citation Download Citation

MingChang Shih, Chang Ten Lian, and J. S. Hwang "Application of phase contrast microscopy to the study of marine microbiota", Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); https://doi.org/10.1117/12.390552

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