Polyaniline-coated nanospray emitters: a route to low-flow separations mass spectrometry

Troy D. Wood; Sarah A. Lorenz; E. Peter Maziarz III

doi:10.1117/12.380497

22 March 2000 Polyaniline-coated nanospray emitters: a route to low-flow separations mass spectrometry

Troy D. Wood, Sarah A. Lorenz, E. Peter Maziarz III

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380497
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Nanoelectrospray or nanospray ionization has revolutionized biological mass spectrometry because of its low sample consumption requirements, low flow rates (nL/min), and improved ion transfer efficiency from source to detector versus conventional electrospray ionization. These advantages suggest that nanospray could prove beneficial in the coupling to on-line capillary separations methods, such as capillary electrophoresis and capillary electrochromatography. In order to establish a stable spray, the nanospray emitter must be electrically biased relative to a counter-electrode inlet to the mass spectrometer, this is most easily achieved by coating the nanospray emitter with a conductive metal coating. However, metallized nanospray tips are highly susceptible to deterioration by electrical discharge. Due to this limited lifetime, nanospray emitters are limited for coupling to capillary separations techniques. Here, an alterative coating for nanospray emitters using polyaniline is demonstrated.

Citation Download Citation

Troy D. Wood, Sarah A. Lorenz, and E. Peter Maziarz III "Polyaniline-coated nanospray emitters: a route to low-flow separations mass spectrometry", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380497

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