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A spectrophotometric method optical density ratio for simultaneous determination of four derivatives as for deoxyhemoglobin, oxyhemoglobin, carboxyhemoglobin and methemoglobin in a blood sample is described. The normalized averaged spectrum of derivatives hemoglobin in the spectral range 14200 - 21000 cm-1 is reduced.
Olexander I. Bilyi,Mykola M. Veliky, andKateryna P. Dudok
"Original method of concurrent determination of hemoglobin derivatives", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380517
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Olexander I. Bilyi, Mykola M. Veliky, Kateryna P. Dudok, "Original method of concurrent determination of hemoglobin derivatives," Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380517