Light-scattering microscope as a tool to investigate scattering heterogeneity in tissue

Alois K. Popp; Megan T. Valentine; Peter D. Kaplan; David A. Weitz

doi:10.1117/12.382746

19 April 2000 Light-scattering microscope as a tool to investigate scattering heterogeneity in tissue

Alois K. Popp, Megan T. Valentine, Peter D. Kaplan, David A. Weitz

Proceedings Volume 3917, Optical Biopsy III; (2000) https://doi.org/10.1117/12.382746
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Rayleigh light scattering has not yet been used for quantitative investigations of heterogeneous systems. Preconditions such an experiment are a well defined scattering geometry and independent information about the local state of the sample. We have designed a new instrument that meets these criteria: a light-scattering microscope with simultaneous imaging. We demonstrate the ability to characterize local differences within one tissue type as well as global differences between tissue types. Real space images of the sample are taken by normal video microscopy techniques. The light scattering pattern in analyzed by the evaluation of wave-vector dependence and scattering direction of the scattered intensity. Statistical analysis of scattering patterns show what is important for the characterization and classification of tissues and heterogeneous structures. Real space images provide context for scattering analysis. The light scattering microscope is a powerful tool for characterization of local structural order in inhomogeneous structures like tissues.

Citation Download Citation

Alois K. Popp, Megan T. Valentine, Peter D. Kaplan, and David A. Weitz "Light-scattering microscope as a tool to investigate scattering heterogeneity in tissue", Proc. SPIE 3917, Optical Biopsy III, (19 April 2000); https://doi.org/10.1117/12.382746

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