Light activation of the immune system part I: influence on G-class antibodies

Vladimir P. Zharov; Vladimir S. Gevondyan; Natalya M. Gevondyan; Sergey A. Ermilov; Alla M. Volynskaya

doi:10.1117/12.388075

13 June 2000 Light activation of the immune system part I: influence on G-class antibodies

Vladimir P. Zharov, Vladimir S. Gevondyan, Natalya M. Gevondyan, Sergey A. Ermilov, Alla M. Volynskaya

Proceedings Volume 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical; (2000) https://doi.org/10.1117/12.388075
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The experimental evidences for photochemical mechanism of light activation of immune system are presented. The experiments were made in vitro using serums from normal blood, from blood of people with secondary immunodeficiency containing low-avidity IgG (up to 75 - 90%), and pure IgG buffer solutions obtained from the same serums. LED arrays and halogen lamps with narrow spectral filters from UV to IR were used as optical monochromatic sources. We have discovered that light can activate immune system by IgG transformation from low-avidity state to high-avidity one. This change has multistage irreversible character and depends on light wavelength and intensity. The estimations of optical effects were made by determination of IgG functional activity and quaternary antibody structure through the number of accessible functional protein residues in the IgG buffer solutions. Both methods showed very correlated results. Each IgG avidity transformation stage correlated with definite change of antibody spatial structure that, to our opinion, corresponds to the phenomenon pass through the several intermediate metastable forms, which are maintained by different intermolecular bonds. The bioaction spectrum of discovered effects is also presented.

Citation Download Citation

Vladimir P. Zharov, Vladimir S. Gevondyan, Natalya M. Gevondyan, Sergey A. Ermilov, and Alla M. Volynskaya "Light activation of the immune system part I: influence on G-class antibodies", Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); https://doi.org/10.1117/12.388075

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