Hydrodynamic study of the behavior of chondroitin sulphate under nondestructive laser irradiation of cartilage

Emil N. Sobol; Alexander I. Omelchenko; Alexander P. Sviridov; Stephen E. Harding; Kornelia Jumel; Nicholas Jones

doi:10.1117/12.388025

13 June 2000 Hydrodynamic study of the behavior of chondroitin sulphate under nondestructive laser irradiation of cartilage

Emil N. Sobol, Alexander I. Omelchenko, Alexander P. Sviridov, Stephen E. Harding, Kornelia Jumel, Nicholas Jones

Author Affiliations +

Proceedings Volume 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical; (2000) https://doi.org/10.1117/12.388025
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The effects of laser irradiation on molecular mass and conformation of pure chondroitin sulphate dissolved in phosphate buffered saline were investigated using size exclusion chromatography/multi-angle light scattering (SEC/MALS) and sedimentation velocity in the analytical ultracentrifuge. In addition, cartilage pieces immersed in buffer were irradiated with a laser in order to study whether cartilage components may diffuse away from the matrix and into the surrounding aqueous medium as a result of laser treatment. Size exclusion chromatography/multi-angle light scattering and sedimentation velocity measurements showed that (a) laser irradiation decreases the molecular mass of chondroitin sulphate and (b) laser irradiation of cartilage induces diffusion of macromolecules into the medium. The results obtained allow us to understand the mechanism of stress relaxation and structural alterations in cartilage under non- destructive laser radiation.

Citation Download Citation

Emil N. Sobol, Alexander I. Omelchenko, Alexander P. Sviridov, Stephen E. Harding, Kornelia Jumel, and Nicholas Jones "Hydrodynamic study of the behavior of chondroitin sulphate under nondestructive laser irradiation of cartilage", Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); https://doi.org/10.1117/12.388025

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