Novel peptide matrix for immobilization of biomolecules for surface plasmon resonance sensing

Tatiana I. Ksenevich; Sergei I. Rogov; Maxim N. Zmak; Maya A. Kupriyanova; Dmitrii V. Klinov; Sergey A. Grachev; Petr I. Nikitin

doi:10.1117/12.372918

15 December 1999 Novel peptide matrix for immobilization of biomolecules for surface plasmon resonance sensing

Tatiana I. Ksenevich, Sergei I. Rogov, Maxim N. Zmak, Maya A. Kupriyanova, Dmitrii V. Klinov, Sergey A. Grachev, Petr I. Nikitin

Author Affiliations +

Proceedings Volume 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection; (1999) https://doi.org/10.1117/12.372918
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

A novel synthetic peptide spacer designed on a gold film is introduced for use in surface plasmon resonance (SPR) sensing. The peptide was a specially designed sequence of amino acids, synthesized by the Fmoc-solid-phase chemistry. The peptide was adsorbed on the gold film from an aqueous solution via its four thiol groups, forming a self-assembled negatively charged monolayer. The monolayer contained carboxyl groups, which were activated by the EDC/NHS technique. It was successfully used as a matrix (2 - 4 nm thick) for covalent immobilization of fusion protein, which included C-terminal fragment of human 5-hydroxytryptamine transporter (molecular weight approximately 21 kDa). The reaction between the immobilized protein and antibodies was monitored by SPR means. The matrix did not cause degradation of immobilized components and steric hindrances to mass transport, and also demonstrated low nonspecific binding to antibodies. Besides, the matrix could be regenerated without decreasing SPR response to the reaction. Along with the ability to immobilize high weight molecules, which are unable to enter a conventional CM-dextran matrix due to steric hindrances to mass transport, the peptide matrix has a number of advantages over the CM-dextran matrix, namely, simplicity in preparation, low cost, and much shorter time needed for preparation. The peptide spacer matrix can be widely used not only in SPR, but also in other analytical techniques that require immobilization of proteins on metal surfaces, such as interferometry, piezoelectric detection, scanning tunneling microscopy.

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Tatiana I. Ksenevich, Sergei I. Rogov, Maxim N. Zmak, Maya A. Kupriyanova, Dmitrii V. Klinov, Sergey A. Grachev, and Petr I. Nikitin "Novel peptide matrix for immobilization of biomolecules for surface plasmon resonance sensing", Proc. SPIE 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection, (15 December 1999); https://doi.org/10.1117/12.372918

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KEYWORDS

Gold

Proteins

Sensors

Surface plasmons

Atomic force microscopy

Molecules

Matrices

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