AMBRI biosensor: stabilizing artificial membranes and receptor attachment

Chris J. Burns; Vijoleta Braach-Maksvytis; L. G. King; Peter D. J. Osman; Ronald J. Pace; Burkhard Raguse; L. Wieczorek; B. A. Cornell

doi:10.1117/12.372925

15 December 1999 AMBRI biosensor: stabilizing artificial membranes and receptor attachment

Chris J. Burns, Vijoleta Braach-Maksvytis, L. G. King, Peter D. J. Osman, Ronald J. Pace, Burkhard Raguse, L. Wieczorek, B. A. Cornell

Author Affiliations +

Proceedings Volume 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection; (1999) https://doi.org/10.1117/12.372925
Event: Photonics East '99, 1999, Boston, MA, United States

Abstract

The AMBRI Ion Channel Switch biosensor is a novel scanning technology with broad application in a variety of fields. The technology is based on an artificial bilayer membrane attached to gold through hydrophilic tethers. The lamellar bilayer membrane possesses electrical characteristics similar to black (bilayer) lipid membranes being sealed with a capacitance of approximately 0.6 (mu) F/cm², is fluid, and is stable to a variety of media including plasma and whole blood and to challenges with solvent solutions. Receptors/antibodies can be attached to the membrane through biotin-streptavidin linkages, and use of caged biotin species allows optical patterning of the membrane surface.

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Chris J. Burns, Vijoleta Braach-Maksvytis, L. G. King, Peter D. J. Osman, Ronald J. Pace, Burkhard Raguse, L. Wieczorek, and B. A. Cornell "AMBRI biosensor: stabilizing artificial membranes and receptor attachment", Proc. SPIE 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection, (15 December 1999); https://doi.org/10.1117/12.372925

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