Evaluation of a red fluorescent dye for high-throughput compound screening applications in pharmaceutical discovery research

Kerry M. Swift; Edmund D. Matayoshi

doi:10.1117/12.347522

3 May 1999 Evaluation of a red fluorescent dye for high-throughput compound screening applications in pharmaceutical discovery research

Kerry M. Swift, Edmund D. Matayoshi

Author Affiliations +

Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV; (1999) https://doi.org/10.1117/12.347522
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

The possibility and potential benefits of using an extreme red fluorescent dye such as Cy5.5^TM to label drug discovery target proteins was studied experimentally. Cy5.5 labeled BSA, GFP, and CRP were used as examples of protein-dye conjugates whose binding to corresponding antibody was detected by changes in either rotational or transnational diffusion properties, that is, by either fluorescence polarization or confocal fluorescence correlation spectroscopy (FCS). In addition, FCS was used to quantitate excitation of Cy5.5 to its triplet state. Fluorescence polarization and lifetime were measured as a function of excitation wavelength or glycerol concentration and solvent viscosity.

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Kerry M. Swift and Edmund D. Matayoshi "Evaluation of a red fluorescent dye for high-throughput compound screening applications in pharmaceutical discovery research", Proc. SPIE 3602, Advances in Fluorescence Sensing Technology IV, (3 May 1999); https://doi.org/10.1117/12.347522

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