Multispectral fluorescence imaging device for malignancy detection

Thomas Bocher; Till Luhmann; S. Baier; Marc Dierolf; M. Naumann; Juergen Beuthan; Hans-Peter Berlien; Gerhard J. Mueller

doi:10.1117/12.297951

29 December 1997 Multispectral fluorescence imaging device for malignancy detection

Thomas Bocher, Till Luhmann, S. Baier, Marc Dierolf, M. Naumann, Juergen Beuthan, Hans-Peter Berlien, Gerhard J. Mueller

Author Affiliations +

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297951
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

In medical diagnosis of superficial lesions at inner or outer surfaces of the human body fluorescence imaging techniques are able to deliver additional information on the metabolic and structural state of the observed tissue. To subtract background fluorescence and to achieve a differential diagnosis a multispectral analysis in several wavelength windows is needed. Additionally, special image algorithms have to be applied which depend on the examined malignancy. For this purpose a multispectral fluorescence imaging device was developed. It can be used both endoscopically and in combination with a standard operational microscope from Carl Zeiss, Germany. In this paper, the device and first clinical results are presented. The device was built to detect superficial lesions like tumors, inflammations, etc. Target chromophores are NADH, Protoporphyrin IX, collagen and other. The measured optical bands are (405 plus or minus 5) nm, (442 plus or minus 5) nm, (458 plus or minus 5) nm, (550 plus or minus 5) nm, (630 plus or minus 5) nm and (690 plus or minus 5) nm. A special UV-source with a liquid light guide is used as the illumination source in two excitation bands of (365 plus or minus 10) nm and (420 plus or minus 20) nm. First clinical investigations of superficial malignancies like squamous cell carcinoma and basalioma are presented.

Citation Download Citation

Thomas Bocher, Till Luhmann, S. Baier, Marc Dierolf, M. Naumann, Juergen Beuthan, Hans-Peter Berlien, and Gerhard J. Mueller "Multispectral fluorescence imaging device for malignancy detection", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297951

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