Laser-induced fluorescence studies of premalignant and benign lesions in the female genital tract

Claes af Klinteberg; Ingrid Wang; Charlotta Lindquist; Aurelija Vaitkuviene; Katarina Svanberg M.D.

doi:10.1117/12.297947

29 December 1997 Laser-induced fluorescence studies of premalignant and benign lesions in the female genital tract

Claes af Klinteberg, Ingrid Wang, Charlotta Lindquist, Aurelija Vaitkuviene, Katarina Svanberg M.D.

Author Affiliations +

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297947
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Laser-induced fluorescence (LIF) was studied in vivo from premalignant and benign lesions in the female genital tract, in particular the cervix. The aim of the study was to investigate the possibilities to differentiate cervical intraepithelial neoplasia (CIN) from normal tissue by means of two different fluorescence modalities. Most of the patients were given a low dose (5 mg/kg bw) of (delta) -amino levulinic acid (ALA). The ALA was orally administered 2 - 4 hours prior to the investigation. During this time, the ALA is transformed to the strongly fluorescent protoporphyrin IX (PpIX) via the haem cycle. Excitation light with a wavelength of 405 nm was used to excite the PpIX fluorescence. Excess amounts of PpIX were accumulated preferentially in diseased tissue. However, the variability in the PpIX accumulation from patient to patient was large. By using excitation light at 337 nm, the endogenous fluorophores are more efficiently excited. Therefore, this excitation modality was exploited for studying spectral characteristics of the autofluorescence in different tissue types. The spectra obtained were evaluated by forming fluorescence intensity ratios. The tissue types were grouped according to the histopathological examination. A correlation with the fluorescence ratios was performed. Some problems with the classification remain, mostly due to the difficulties in obtaining histopathologic evaluation of the biopsies at the exact location of the LIF measurements.

Citation Download Citation

Claes af Klinteberg, Ingrid Wang, Charlotta Lindquist, Aurelija Vaitkuviene, and Katarina Svanberg M.D. "Laser-induced fluorescence studies of premalignant and benign lesions in the female genital tract", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297947

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