Photobiomodulation of vascular endothelial and smooth muscle cells in vitro with red laser light

Nicholas Kipshidze; Michael H. Keelan Jr.; Joseph B. Horn; Victor Nikolaychik

doi:10.1117/12.260692

3 December 1996 Photobiomodulation of vascular endothelial and smooth muscle cells in vitro with red laser light

Nicholas Kipshidze, Michael H. Keelan Jr., Joseph B. Horn, Victor Nikolaychik

Proceedings Volume 2922, Laser Applications in Medicine and Dentistry; (1996) https://doi.org/10.1117/12.260692
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Numerous reports suggest that low power red laser light (LPRLL) is capable of affecting cellular processes in the absence of significant thermal effect. The objective of the present study was to determine the effect of LPRLL on viability, growth, and attachment characteristics of rabbit and human aortic endothelial cells (EC) and smooth muscle cells (SMC) in vitro. All cell cultures were irradiated with single dose LPRLL using a He-Ne continuous wave laser with different energy densities. Assessment of effect on cell viability, growth, and attachment was performed utilizing Alamar Blue assay. Based upon our experiments, we conclude that: 1) stimulation and/or inhibition of cell growth and death can be obtained with LPRLL by varying the energy level, 2) LPRLL increases EC attachment, and 3) EC are more sensitive to photobiomodulation with LPRLL than SMC. These data may have significant importance leading to the establishment of new methods for phototherapy of atherosclerosis and restenosis.

Citation Download Citation

Nicholas Kipshidze, Michael H. Keelan Jr., Joseph B. Horn, and Victor Nikolaychik "Photobiomodulation of vascular endothelial and smooth muscle cells in vitro with red laser light", Proc. SPIE 2922, Laser Applications in Medicine and Dentistry, (3 December 1996); https://doi.org/10.1117/12.260692

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