Zona pellucida drilling by a 1.48-um laser: influence on the biomechanics of the hatching process

Klaus Rink; Laurent Descloux; Guy P. Delacretaz; Alfred Senn; Dorotha Nocera; Marc Germond

doi:10.1117/12.229569

10 January 1996 Zona pellucida drilling by a 1.48-um laser: influence on the biomechanics of the hatching process

Klaus Rink, Laurent Descloux, Guy P. Delacretaz, Alfred Senn, Dorotha Nocera, Marc Germond

Proceedings Volume 2624, Laser-Tissue Interaction and Tissue Optics; (1996) https://doi.org/10.1117/12.229569
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Efficient and well controlled laser drilling of the zona pellucida of mouse eggs has been demonstrated recently using a 1.48 micrometers laser diode. The zona pellucida openings (5 - 10 micrometers ) can be obtained within the culture dish in an either tangential or more poleward irradiation in respect to the spherical egg structure. Zone drilling is achieved at a laser power of 47 mW and typical irradiation times of 8 - 20 ms for mouse oocytes. Cultured mouse embryos evidence strongly modified hatching behavior when drilled. No increase of the outer diameter of the zona pellucida and no thinning are induced prior to hatching in the lased group, in contrast to the control non-drilled group. Drilled embryos hatches one day earlier than control non-drilled embryos. Obviously no mechanical constraint is induced on the zona pellucida during the initial development of the drilled blastocysts before hatching and hatching is facilitated.

Citation Download Citation

Klaus Rink, Laurent Descloux, Guy P. Delacretaz, Alfred Senn, Dorotha Nocera, and Marc Germond "Zona pellucida drilling by a 1.48-um laser: influence on the biomechanics of the hatching process", Proc. SPIE 2624, Laser-Tissue Interaction and Tissue Optics, (10 January 1996); https://doi.org/10.1117/12.229569

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