Immobilization of antibodies onto gold and titanium oxide surfaces

Marie-Claude Millot; Francoise Martin; Juliette Omont; Bernard Sebille; Yves Levy

doi:10.1117/12.221728

29 September 1995 Immobilization of antibodies onto gold and titanium oxide surfaces

Marie-Claude Millot, Francoise Martin, Juliette Omont, Bernard Sebille, Yves Levy

Proceedings Volume 2508, Chemical, Biochemical, and Environmental Fiber Sensors VII; (1995) https://doi.org/10.1117/12.221728
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany

Abstract

The immobilization of antibodies onto surfaces by means of a reactive polymer has been studied. Two types of surfaces, gold and titanium oxide, were investigated. After modification with a suitable reagent bearing amino group, these surfaces are able to link covalently the reactive polymer through its N-hydroxysuccinimide carbonate functions. After polymer immobilization, a reaction between antibody and the remaining reactive sites on the polymer can take palce. This permits a covalent binding of biomolecules to the surface. The resulting biosensor is stable and specific. Another approach has been used for immobilization of antibodies: it involves a modification of the polymer with sulfonate groups and fixation of tetravalent metal ions. The resulting biosensor can recognize specifically the corresponding antigen and the regeneration of the surface between polymer and antibody layers is possible.

Citation Download Citation

Marie-Claude Millot, Francoise Martin, Juliette Omont, Bernard Sebille, and Yves Levy "Immobilization of antibodies onto gold and titanium oxide surfaces", Proc. SPIE 2508, Chemical, Biochemical, and Environmental Fiber Sensors VII, (29 September 1995); https://doi.org/10.1117/12.221728

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