PDT-induced apoptosis in arterial smooth muscles cells

Isaac Nyamekye; R. Renick; C. Gilbert; Jean R. McEwan; G. Evan; Christopher C. R. Bishop; Stephen G. Bown

doi:10.1117/12.203419

1 March 1995 PDT-induced apoptosis in arterial smooth muscles cells

Isaac Nyamekye, R. Renick, C. Gilbert, Jean R. McEwan, G. Evan, Christopher C. R. Bishop, Stephen G. Bown

Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203419
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States

Abstract

PDT kills smooth muscle cells (SMC) in vivo and thus prevents intimal hyperplasia after angioplasty. It causes little inflammation and structural integrity of the artery is not compromised. We have studied the process of the SMC death in vitro. Cultured rat SMC (cell line sv40 ATCC) were sensitized with aluminum disulphonated phthalocyanine (AlS₂Pc), and then irradiated with 675 nm laser light (2.5 J/cm²). Controls were studied using only sensitizer or laser for treatment. The cells were incubated and the dying process observed with a time lapse video and microscope system. PDT caused a characteristic pattern of death. Cells lost contact with neighbors, shrank, and showed hyperactivity and membrane ruffling. The cells imploded into active and condensed membrane bound vesicles which were terminally reduced to residual bodies. These are the morphological changes of apoptosis. The control cells which were given AlS₂Pc alone or laser alone showed no death. PDT induced cultured arterial SMC death by apoptosis rather than necrosis. An apoptotic mechanism of cell death in vivo would explain the relative lack of inflammation and local tissue destruction in the face of massive death.

Citation Download Citation

Isaac Nyamekye, R. Renick, C. Gilbert, Jean R. McEwan, G. Evan, Christopher C. R. Bishop, and Stephen G. Bown "PDT-induced apoptosis in arterial smooth muscles cells", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203419

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