Manipulation and characterization of cells by laser light

Jan Greve; Richard M.P. Doornbos; Tom C. Bakker Schut; Bart G. de Grooth

doi:10.1117/12.146190

17 June 1993 Manipulation and characterization of cells by laser light

Jan Greve, Richard M.P. Doornbos, Tom C. Bakker Schut, Bart G. de Grooth

Proceedings Volume 1922, Laser Study of Macroscopic Biosystems; (1993) https://doi.org/10.1117/12.146190
Event: Laser Spectroscopy of Biomolecules: 4th International Conference on Laser Applications in Life Sciences, 1992, Jyvaskyla, Finland

Abstract

Both for medical and biotechnological purposes it is very important to be able to analyze and manipulate cellular populations on a single cell basis. Moreover it is often important to be able to sort a subfraction of cells from the total population with defined specific characteristics. Since the retainment of biological viability is of importance in such cases the use of monoclonal antibodies for cell characterization cannot always be applied. The use of light scattering offers an attractive alternative. One of the manipulations which is at the beginning of many biotechnological processes is the fusion between two well characterized and differing cell types. In the present paper we concentrate on the use of flowcytometry for light scattering characterization and fusion of cells.

Citation Download Citation

Jan Greve, Richard M.P. Doornbos, Tom C. Bakker Schut, and Bart G. de Grooth "Manipulation and characterization of cells by laser light", Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); https://doi.org/10.1117/12.146190

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