Accuracy of two-color peak-height-encoded DNA sequencing by capillary gel electrophoresis and laser-induced fluorescence

Sue Bay; Heather Starke; John Elliott; Norman J. Dovichi

doi:10.1117/12.146713

24 June 1993 Accuracy of two-color peak-height-encoded DNA sequencing by capillary gel electrophoresis and laser-induced fluorescence

Sue Bay, Heather Starke, John Elliott, Norman J. Dovichi

Author Affiliations +

Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146713
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The two-color peak-height encoded DNA sequencing technique was evaluated with six clones taken from the malaria genome. This technique produced a sequencing accuracy of at least 97.5%. Capillary gel electrophoresis, using a modest voltage of 200 V/cm, showed a three- fold increase in speed and higher efficiency compared to conventional slab gel technique.

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Sue Bay, Heather Starke, John Elliott, and Norman J. Dovichi "Accuracy of two-color peak-height-encoded DNA sequencing by capillary gel electrophoresis and laser-induced fluorescence", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146713

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