Three-dimensional confocal microscopy and visualization

Barry R. Masters

doi:10.1117/12.131125

22 September 1992 Three-dimensional confocal microscopy and visualization

Barry R. Masters

Author Affiliations +

Proceedings Volume 1808, Visualization in Biomedical Computing '92; (1992) https://doi.org/10.1117/12.131125
Event: Visualization in Biomedical Computing, 1992, Chapel Hill, NC, United States

Abstract

This tutorial provides a fundamental introduction to confocal microscopy and its applications. The course will review the historical developments of confocal microscopy and describe all of the components of confocal microscopes, including light sources, scanning systems, microscope objectives, apertures, detection systems, and mechanical xyz stages. A comparison between ideal and real optical systems will be developed, with "focus" on optical aberrations and digital deconvolutions.

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Barry R. Masters "Three-dimensional confocal microscopy and visualization", Proc. SPIE 1808, Visualization in Biomedical Computing '92, (22 September 1992); https://doi.org/10.1117/12.131125

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