Fluorescence photometric analysis of Photofrin uptake and the detection of precancerous and true epidermoid lesions

Thomas S. Mang; David H. Crean; Frank T. Sindoni; Charles Liebow

doi:10.1117/12.59352

6 May 1992 Fluorescence photometric analysis of Photofrin uptake and the detection of precancerous and true epidermoid lesions

Thomas S. Mang, David H. Crean, Frank T. Sindoni, Charles Liebow

Author Affiliations +

Proceedings Volume 1641, Physiological Monitoring and Early Detection Diagnostic Methods; (1992) https://doi.org/10.1117/12.59352
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Neoplastic tissue can be detected by its increased fluorescence compared to surrounding normal tissue after the injection of the tumor-localizing compound Photofrin®. In vivo fluorescence photometry is a non-imaging photodetector which detects the 690 nm fluorescence of the porphyrin. The sensitivity of the instrumentation has allowed the detection of micrometastases in both pre-clinical and clinical studies using low, non-photosensitizing levels of the drug. The technique is now being applied to the 9,10 dimethyl-1,2-.benzanthracene (DMBA)induced hamster buccal cheek pouch carcinoma model to obtain data on the correlation between Photofrmn® uptake and tumor development. This model shows consistent time patterns of tumor development as well precancerous leukoplakia lesions and has been well documented as an animal model of oral epidermoid carcinogenesis. The buccal cheek pouches of Syrian Golden hamsters were exposed to a 0.5% DMBA in acetone thrice weekly for specified time durations. Hamsters were subsequently injected with 1.0 mgfkg of Photofrmn® within the various stages of tumor development. Twenty-four hours post-injection, fluorescence due to drug uptake was measured by in vivo fluorescence photometry. Mucosal tissues were subsequently biopsied and used for extraction assays. Results demonstrate that Photoflin® is retained in DMBA treated tissue with a linear relationship between length of application and Photofrin® uptake and fluorescence. This relationship establishes that premalignant lesions can be distinguished from normal tissue by Photofrmn® uptake and fluorescence and suggest that Photofrmn® uptake and fluorescence can be used in a predictive manner to diagnose and determine the progression of individual lesions.

Citation Download Citation

Thomas S. Mang, David H. Crean, Frank T. Sindoni, and Charles Liebow "Fluorescence photometric analysis of Photofrin uptake and the detection of precancerous and true epidermoid lesions", Proc. SPIE 1641, Physiological Monitoring and Early Detection Diagnostic Methods, (6 May 1992); https://doi.org/10.1117/12.59352

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Tissue optics

Tissues

Tumors

Cancer

Tumor growth modeling

Photometry

Show All Keywords

Keywords/Phrases

Search In:

Publication Years