Theoretical considerations for evanescent-wave immunosensors in biomedical applications

Donna S. Orvedahl; Walter F. Love; Rudolf E. Slovacek

doi:10.1117/12.56545

1 March 1992 Theoretical considerations for evanescent-wave immunosensors in biomedical applications

Donna S. Orvedahl, Walter F. Love, Rudolf E. Slovacek

Proceedings Volume 1587, Chemical, Biochemical, and Environmental Fiber Sensors III; (1992) https://doi.org/10.1117/12.56545
Event: OE Fiber, 1991, Boston, MA, United States

Abstract

Using evanescent wave immunosensors, a fluorescent labeled analyte may be concentrated within the active surface region by a combination of diffusion to the fiber surface and trapping of the molecule or complex by an antigen-antibody affinity reaction. With the dye B- phycoerythrin, approximately 1.5 X 10-22 moles may be sensed over a 1 mm2 surface. From this number (as determined for a particular dye and instrumentation system) and antibody affinity constants, limits to assay sensitivity can be calculated and the kinetics modeled.

Citation Download Citation

Donna S. Orvedahl, Walter F. Love, and Rudolf E. Slovacek "Theoretical considerations for evanescent-wave immunosensors in biomedical applications", Proc. SPIE 1587, Chemical, Biochemical, and Environmental Fiber Sensors III, (1 March 1992); https://doi.org/10.1117/12.56545

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