Development of a novel in-vivo drug/in-vitro light system to investigate mechanisms of cell killing with photodynamic therapy

James A. Hampton; Steven H. Selman M.D.

doi:10.1117/12.44052

1 June 1991 Development of a novel in-vivo drug/in-vitro light system to investigate mechanisms of cell killing with photodynamic therapy

James A. Hampton, Steven H. Selman M.D.

Author Affiliations +

Proceedings Volume 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques; (1991) https://doi.org/10.1117/12.44052
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

Over the last decade considerable interest has developed in the use of exogenously administered chromophores in combination with visible light for the treatment of human tumors. Whether cells are killed directly, or indirectly as a result of disruption of the tissue, microvasculature is unknown. The authors have developed methods to assess in short term culture the effects of PDT on precision cut tissue slices. The use of these tissue slices provide an important link between in vivo studies and in vitro isolated cultured cells for the following reasons: 1) slices contain all of the normal cells in their proper in vivo architectural arrangement; 2) since slices can be obtained relatively easily and in a very short period of time (a few minutes), animals can be treated with compounds in vivo, the tissue can be removed, sliced and mechanistic studies performed in vitro (without the several hours delay required to produce cultured cells); 3) in vitro comparisons between species, including human, can be readily made; and 4) mechanisms of PDT-induced cell killing can be studied in the absence of a functioning vascular system. Using this in vivo drug/in vitro light system, the results presented will detail the findings using normal rat liver and a transplantable rat tumor model.

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James A. Hampton and Steven H. Selman M.D. "Development of a novel in-vivo drug/in-vitro light system to investigate mechanisms of cell killing with photodynamic therapy", Proc. SPIE 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques, (1 June 1991); https://doi.org/10.1117/12.44052

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