Quantitative phase imaging through scattering layer by lensless digital holographic microscopy

Xingpeng Du; Vinu R. V.; Ziyang Chen; Jixiong Pu

doi:10.1117/12.3036411

22 November 2024 Quantitative phase imaging through scattering layer by lensless digital holographic microscopy

Xingpeng Du, Vinu R. V., Ziyang Chen, Jixiong Pu

Proceedings Volume 13240, Holography, Diffractive Optics, and Applications XIV; 132401A (2024) https://doi.org/10.1117/12.3036411
Event: SPIE/COS Photonics Asia, 2024, Nantong, Jiangsu, China

Abstract

The label-free computational techniques with phase imaging capability unfolds the domain of quantitative phase imaging (QPI) to a broad range of microscopy applications. However, the presence of scattering layers in the propagation path makes the conventional QPI approaches susceptible due to the light scattering effects in the medium. Owing to this phase imaging challenge through scattering layers, we propose and experimentally demonstrate a technique for the quantitative imaging through scattering layers by integrating the lens-less digital holographic microscopy with speckle correlation approach. The proposed technique utilizes a holographic compact microscopy scheme for the recovery of phase information of the object located at any arbitrary planes behind the scattering medium by utilizing a correlation hologram and an in-line hologram. The QPI capability of the developed system is theoretically introduced and experimentally validated by imaging various standard and unknown phase samples.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Xingpeng Du, Vinu R. V., Ziyang Chen, and Jixiong Pu "Quantitative phase imaging through scattering layer by lensless digital holographic microscopy", Proc. SPIE 13240, Holography, Diffractive Optics, and Applications XIV, 132401A (22 November 2024); https://doi.org/10.1117/12.3036411

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