Non-contact molecular specific transient absorption microscopy

Ardalan Farazmand; Scott P. Mattison

doi:10.1117/12.3034718

21 October 2024 Non-contact molecular specific transient absorption microscopy

Ardalan Farazmand, Scott P. Mattison

Proceedings Volume 13186, SPIE Translational Biophotonics + Additive Manufacturing for Photonics 2024; 1318618 (2024) https://doi.org/10.1117/12.3034718
Event: SPIE Translational Biophotonics + Additive Manufacturing for Photonics, 2024, Houston, Texas, United States

Conference Poster

Abstract

Monitoring dynamic activity of biochemical pathways is an essential, but challenging, step for diagnosing, treating, and understanding human pathologies. Transient absorption is a resonant two-photon optical imaging technique capable of probing multiple molecular-specific properties, such as ground state recovery time, absorption spectra, and transient absorption spectra, all with high resolution. Previously, photoacoustic based transient absorption imaging approaches have demonstrated the ability to probe ground state recovery time of biomolecules and provide spatial contrast on par with confocal microscopy. A fundamental challenge of these approaches relied on overlapping the focus of an ultrasound transducer with the optical focus for image detection, making the technique challenging to perform in vitro or in vivo. Here, we present non-contact methods for detecting transient absorption via the photoacoustic effect. These methods not only circumvent the need for ultrasound transducers but also enable integration with existing fluorescence imaging techniques.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Ardalan Farazmand and Scott P. Mattison "Non-contact molecular specific transient absorption microscopy", Proc. SPIE 13186, SPIE Translational Biophotonics + Additive Manufacturing for Photonics 2024, 1318618 (21 October 2024); https://doi.org/10.1117/12.3034718

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