Label-free sample analysis using digital holographic microscopy

Aswathy Vijay; Manjoosha Y. R.; Jyothika V. G.; Renu John

doi:10.1117/12.3028914

17 April 2024 Label-free sample analysis using digital holographic microscopy

Aswathy Vijay, Manjoosha Y. R., Jyothika V. G., Renu John

Proceedings Volume 13108, Women in Optics and Photonics in India 2023; 1310802 (2024) https://doi.org/10.1117/12.3028914
Event: Women in Optics and Photonics in India 2023, 2024, Chennai, India

Conference Poster

Abstract

Digital holographic microscopy (DHM) is a label- free quantitative imaging technique to image and characterize translucent to transparent samples. It uses coherent principles of the light source for interferometric detection of transparent and semi- transparent samples. The coherence property of the laser source leads to speckles which often act as noise. Laser speckle contrast imaging is a non-destructive tool for full field mapping of flow in the field of view. It can be integrated with off-axis DHM for applications in optofluidics. This enables the mapping of flow within a microfluidic channel and quantifying the channel dimensions as well as the particles flowing through the channel.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Aswathy Vijay, Manjoosha Y. R., Jyothika V. G., and Renu John "Label-free sample analysis using digital holographic microscopy", Proc. SPIE 13108, Women in Optics and Photonics in India 2023, 1310802 (17 April 2024); https://doi.org/10.1117/12.3028914

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