Comparison of soft tissue handling protocols for identifying optical properties and morphology

Freja Høier; Gavrielle R. Untracht; Amanda Øster Andersen; Karina Stræde; Andreas Kjær; Peter E. Andersen

doi:10.1117/12.3017638

20 June 2024 Comparison of soft tissue handling protocols for identifying optical properties and morphology

Freja Høier, Gavrielle R. Untracht, Amanda Øster Andersen, Karina Stræde, Andreas Kjær, Peter E. Andersen

Author Affiliations +

Proceedings Volume 13006, Biomedical Spectroscopy, Microscopy, and Imaging III; 130060K (2024) https://doi.org/10.1117/12.3017638
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Sample handling is an important consideration when aiming for replicating in vivo conditions ex vivo for the sake of validating imaging protocols and identifying biomarkers of disease. We tested five different handling methods: snap frozen in isopentane, directly frozen at -80°C, slowly frozen in a cryobox with and without cryopreservation media, and formalin fixed. The samples were imaged using optical coherence tomography (OCT) for qualitative and quantitative validation based on morphological features and optical properties. All handling methods were compared to fresh tissue samples using OCT-derived optical properties and morphological features. The results indicate a significant difference in the optical attenuation coefficient as well as morphological differences between the five different methods and support the hypothesis that proper sample handling is crucial for obtaining translatable results.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Freja Høier, Gavrielle R. Untracht, Amanda Øster Andersen, Karina Stræde, Andreas Kjær, and Peter E. Andersen "Comparison of soft tissue handling protocols for identifying optical properties and morphology", Proc. SPIE 13006, Biomedical Spectroscopy, Microscopy, and Imaging III, 130060K (20 June 2024); https://doi.org/10.1117/12.3017638

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