Image flow-cytometry meets virtual reality: a new scenario for label-free quantitative phase contrast microscopy and single cell analysis

Vittorio Bianco; Massimo D'Agostino; Daniele Pirone; Giusy Giugliano; Nicola Mosca; Maria Di Summa; Pasquale Memmolo; Lisa Miccio; Tommaso Russo; Ettore Stella; Pietro Ferraro

doi:10.1117/12.3022945

18 June 2024 Image flow-cytometry meets virtual reality: a new scenario for label-free quantitative phase contrast microscopy and single cell analysis

Vittorio Bianco, Massimo D'Agostino, Daniele Pirone, Giusy Giugliano, Nicola Mosca, Maria Di Summa, Pasquale Memmolo, Lisa Miccio, Tommaso Russo, Ettore Stella, Pietro Ferraro

Author Affiliations +

Proceedings Volume 12997, Optics and Photonics for Advanced Dimensional Metrology III; 1299705 (2024) https://doi.org/10.1117/12.3022945
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Recently, tomographic flow-cytometry in label-free modality has been demonstrated. The tomographic apparatus operates in Quantitative phase imaging (QPI) mode and gives the possibility to retrieve the 3D refractive index distribution of single cells flowing along a microfluidic channel. One of the challenging topic correlated to QPI is the need to extract subcellular compartments as QPI lacks the typical specificity guaranteed by standard fluorescence microscopy (FM). Here we show the possibility of retrieving the specific refractive index distribution of multiple sub-cellular organelles from the 3D tomograms of flowing cells. Furthermore, we show a novel model of representation and fruition of 3D tomograms, displayed in an immersive virtual reality (VR) environment. Thus, new scenarios can be opened not only for fruition but also for analyzing the quantitative measurements of the whole 3D structure of any and each cell.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Vittorio Bianco, Massimo D'Agostino, Daniele Pirone, Giusy Giugliano, Nicola Mosca, Maria Di Summa, Pasquale Memmolo, Lisa Miccio, Tommaso Russo, Ettore Stella, and Pietro Ferraro "Image flow-cytometry meets virtual reality: a new scenario for label-free quantitative phase contrast microscopy and single cell analysis", Proc. SPIE 12997, Optics and Photonics for Advanced Dimensional Metrology III, 1299705 (18 June 2024); https://doi.org/10.1117/12.3022945

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