Oblique plane microscopy-based single molecule localization microscopy (obSTORM) shows promise for superresolution imaging in thick biological samples. However, the Gaussian point spread function (PSF) model used in previous studies limits imaging resolution and axial localization range in obSTORM due to poor fitting with actual PSF shapes. To overcome these limitations, we employed cubic splines to construct a more precise PSF model. This refined model enhances three-dimensional (3D) localization precision, improving obSTORM imaging of mouse retina tissues. It increases imaging resolution by approximately 1.2 times, enables seamless stitching of single molecules across optical sections, and doubles the sectional interval in volumetric obSTORM imaging by extending the usable section thickness. The cubic spline PSF model offers a promising approach for achieving faster and more accurate volumetric obSTORM imaging of biological specimens.
|