Saturated-excitation image scanning microscopy for super-resolution fluorescence imaging

Kenta Temma; Ryosuke Oketani; René Lachmann; Toshiki Kubo; Nicholas I. Smith; Rainer Heintzmann; Katsumasa Fujita

doi:10.1117/12.3007909

20 September 2023 Saturated-excitation image scanning microscopy for super-resolution fluorescence imaging

Kenta Temma, Ryosuke Oketani, René Lachmann, Toshiki Kubo, Nicholas I. Smith, Rainer Heintzmann, Katsumasa Fujita

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 126080T (2023) https://doi.org/10.1117/12.3007909
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

Saturated-excitation (SAX) microscopy can provide theoretically unlimited improvement of spatial resolution in laser scanning microscopy. However, in practice, the signal-to-noise ratio (SNR) limits its capability. In this research, we introduced image scanning microscopy (ISM) into SAX microscopy to improve the SNR.

Citation Download Citation

Kenta Temma, Ryosuke Oketani, René Lachmann, Toshiki Kubo, Nicholas I. Smith, Rainer Heintzmann, and Katsumasa Fujita "Saturated-excitation image scanning microscopy for super-resolution fluorescence imaging", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 126080T (20 September 2023); https://doi.org/10.1117/12.3007909

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