Paper
20 September 2023 Combination of two in-line digital holograms for high accuracy nanoparticles tracking
Anh Tien Dinh, Tuan Duc Pham, Dat Thanh Ha Pham, Yoshio Hayasaki, Quang Duc Pham
Author Affiliations +
Abstract
We propose a new low coherence digital in-line holographic microscope (DIHM) system to accurately obtain the three-dimensional (3D) position of the nanoparticle. The two low coherence digital in-line holograms of the nanoparticle are recorded with two respective illumination angles of the light source. The reconstruction process is carried out with two holograms at different distances to calculate the diffraction images. The 3D position of the nanoparticle can be determined by the diffraction distance where two diffraction images of the nanoparticle form the smallest overlap area. The method can be employed to accurately track the position of the nanoparticles without pre-preparation nanoparticle pattern and also not depending on the structure of the nanoparticle.
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Anh Tien Dinh, Tuan Duc Pham, Dat Thanh Ha Pham, Yoshio Hayasaki, and Quang Duc Pham "Combination of two in-line digital holograms for high accuracy nanoparticles tracking", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 1260806 (20 September 2023); https://doi.org/10.1117/12.3006567
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KEYWORDS
Nanoparticles

Holograms

Diffraction

Digital holography

Light sources

Mirrors

Image processing

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