Light-induced acceleration of antigen-antibody reaction for detecting attogram-level proteins

Takuya Iida; Shota Hamatani; Yumiko Takagi; Kana Fujiwara; Mamoru Tamura; Shiho Tokonami

doi:10.1117/12.3008371

20 September 2023 Light-induced acceleration of antigen-antibody reaction for detecting attogram-level proteins

Takuya Iida, Shota Hamatani, Yumiko Takagi, Kana Fujiwara, Mamoru Tamura, Shiho Tokonami

Proceedings Volume 12606, Optical Manipulation and Structured Materials Conference; 1260618 (2023) https://doi.org/10.1117/12.3008371
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

In this contribution, we reveal a mechanism of light-induced acceleration of antigen-antibody reaction by enhancing the collisions of probe particles and target proteins under the optical and fluidic pressures in microchannel, which leads to the attogram-level detection. The clarified mechanism enables the selective detection of 10¹-10² ag target proteins in sub-microlitter liquid sample with 1-2 orders higher sensitivity and ultrafast specific detection only by 3 minutes of laser irradiation in the absence of any pretreatment procedures in comparison with a conventional immunoassay requiring a few hours. The obtained results will facilitate the development of proteomics and innovative platforms for a variety of biochemical reactions leading to the early diagnosis of various diseases (cancer, dementia, and microbial infections etc).

Citation Download Citation

Takuya Iida, Shota Hamatani, Yumiko Takagi, Kana Fujiwara, Mamoru Tamura, and Shiho Tokonami "Light-induced acceleration of antigen-antibody reaction for detecting attogram-level proteins", Proc. SPIE 12606, Optical Manipulation and Structured Materials Conference, 1260618 (20 September 2023); https://doi.org/10.1117/12.3008371

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