Stimulated Raman scattering microscope for leukemic cell imaging

Cássia Corso Silva; Łukasz Zinkiewicz; Mateusz Pielach; Agnieszka Jamrozik; Milena Królikowska; Joanna Purzycka; Piotr Wasylczyk; Katarzyna Krupa; Yuriy Stepanenko

doi:10.1117/12.2647013

16 March 2023 Stimulated Raman scattering microscope for leukemic cell imaging

Cássia Corso Silva, Łukasz Zinkiewicz, Mateusz Pielach, Agnieszka Jamrozik, Milena Królikowska, Joanna Purzycka, Piotr Wasylczyk, Katarzyna Krupa, Yuriy Stepanenko

Author Affiliations +

Proceedings Volume 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; 123910M (2023) https://doi.org/10.1117/12.2647013
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present a stimulated Raman scattering (SRS) microscope integrated with a novel fiber-based light source. Our light source provides two synchronized pulse trains with 100 mW average power each, independently tunable in the range of 913 to 930 nm and 1024 to 1034 nm, respectively, thus enabling SRS measurements across the 990 to 1300 cm^-1 spectral range with a spectral resolution of 15 cm^-1. We demonstrate the SRS imaging of leukemic cells recorded in a few seconds. Our system may find potential application in biomedicine, in particular, helping to accelerate the diagnostics and follow-up treatment of leukemia patients.

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Cássia Corso Silva, Łukasz Zinkiewicz, Mateusz Pielach, Agnieszka Jamrozik, Milena Królikowska, Joanna Purzycka, Piotr Wasylczyk, Katarzyna Krupa, and Yuriy Stepanenko "Stimulated Raman scattering microscope for leukemic cell imaging", Proc. SPIE 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, 123910M (16 March 2023); https://doi.org/10.1117/12.2647013

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