Concurrent execution of phase compensation and automatic focusing procedures for telecentric off-axis digital holographic microscopy

R. Castañeda; C. Trujillo; A. Doblas

doi:10.1117/12.2652631

16 March 2023 Concurrent execution of phase compensation and automatic focusing procedures for telecentric off-axis digital holographic microscopy

R. Castañeda, C. Trujillo, A. Doblas

Author Affiliations +

Proceedings Volume 12389, Quantitative Phase Imaging IX; 123890A (2023) https://doi.org/10.1117/12.2652631
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

This contribution presents a joint phase compensation and autofocusing method for telecentric off-axis Digital Holographic Microscopy (DHM). Current challenges of off-axis DHM systems applied to in-vivo imaging are the automatic reconstruction of phase images without phase distortions while the specimens under research move within the volume, generating out-of-focus holograms. Although different proposals to tackle these challenges individually have been reported for static samples, in this proposal, both issues are solved concurrently with no additional user intervention. As a result, in-focus compensated phase images of the out-of-focus studied samples are obtained. The proposal has been validated using simulated data.

Conference Presentation

Citation Download Citation

R. Castañeda, C. Trujillo, and A. Doblas "Concurrent execution of phase compensation and automatic focusing procedures for telecentric off-axis digital holographic microscopy", Proc. SPIE 12389, Quantitative Phase Imaging IX, 123890A (16 March 2023); https://doi.org/10.1117/12.2652631

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