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Life sciences are devoted to the research of all aspects of living organisms. As the basic unit of a living organism, biological cells are diverse and different. These differences are a direct reflection of their corresponding biological characteristics, so "seeing the cell more clearly" has been the goal of scientists at home and abroad. Based on the principle of coherent imaging, digital holographic quantitative phase microscopy imaging technology adopts digital recording and numerical reconstruction to realize phase retrieval and three-dimensional reconstruction. It has become one of the most promising methods in cellular research with better imaging efficiency, reconstruction accuracy, and stability. However, in the band-pass filtering process, tilt aberrations are often introduced due to manual frame selection of the +1 order spectrum, which is detrimental to the imaging quality of the system. To address this problem, this paper proposes a method to automatically determine the spectral center based on the surface fitting of a two-dimensional Hann window function, which improves the phase retrieval accuracy and imaging quality without reducing the computational speed. A more accurate spectral center can be obtained by conducting the sub-pixel spectral center of surface fitting. In addition, the accuracy is sub-pixel level and requires sub-pixel displacement correction of the tilted phase plane to obtain better quantitative phase retrieval results and imaging quality.
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