Showing differences in viscoelastic properties of cells growing on micropattern by using very long-time high speed microrheology as a new way to measure cell mechanics

Carolin Grandy; Jonas Pfeil; Fabian Port; Kay-Eberhard Gottschalk; Othmar Marti

doi:10.1117/12.2608158

2 March 2022 Showing differences in viscoelastic properties of cells growing on micropattern by using very long-time high speed microrheology as a new way to measure cell mechanics

Carolin Grandy, Jonas Pfeil, Fabian Port, Kay-Eberhard Gottschalk, Othmar Marti

Author Affiliations +

Proceedings Volume 11971, High-Speed Biomedical Imaging and Spectroscopy VII; 119710B (2022) https://doi.org/10.1117/12.2608158
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Cells react highly sensitive to mechanical and structural influences of their environment by mechanosensing. Micropatterning allows the precise regulation of forces by changing the shape of the cell. In order to record the adaptation to the pattern shape and the changes in viscoelastic properties in detail and over a longer period of time, the VELOMIR sensor is ideally suited. The live cell measurements were recorded at 3000 frames per second over 10 minutes. Here we show the difference in passive microrheology measurements of mouse embryonic fibroblasts with incorporated 1 μm polystyrene beads cultured on three differently patterned shapes in a range of sizes.

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Carolin Grandy, Jonas Pfeil, Fabian Port, Kay-Eberhard Gottschalk, and Othmar Marti "Showing differences in viscoelastic properties of cells growing on micropattern by using very long-time high speed microrheology as a new way to measure cell mechanics", Proc. SPIE 11971, High-Speed Biomedical Imaging and Spectroscopy VII, 119710B (2 March 2022); https://doi.org/10.1117/12.2608158

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