Set-ups to induce by light a memory in in-vitro neuronal cultures

Clara Zaccaria; Mattia Mancinelli; Beatrice Vignoli; Ilya Auslender; Paolo Bettotti; Asiye Malkoç; Marco Canossa; Lorenzo Pavesi

doi:10.1117/12.2606876

7 March 2022 Set-ups to induce by light a memory in in-vitro neuronal cultures

Clara Zaccaria, Mattia Mancinelli, Beatrice Vignoli, Ilya Auslender, Paolo Bettotti, Asiye Malkoç, Marco Canossa, Lorenzo Pavesi

Author Affiliations +

Proceedings Volume 11947, Optogenetics and Optical Manipulation 2022; 1194703 (2022) https://doi.org/10.1117/12.2606876
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

To study the brain and the related neuronal network activity, many attempts were made to design and develop platforms able to induce and record neuronal signals. However, many brain processes - like memory formation and storage - and diseases - like amnesia or epilepsy - need more basic studies. For these, a bottom-up approach is needed, starting from 2D in-vitro neuronal cultures. In this work, we will present two experimental systems able to optogenetically interact with 2D neuronal networks with patternized light. One system consists in a Digital Light Projector (DLP) integrated in a microscope setup, which can illuminate neurons from the top; the other, is a compact and transportable photonic chip, properly designed to illuminate neurons plated on its surface.

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Clara Zaccaria, Mattia Mancinelli, Beatrice Vignoli, Ilya Auslender, Paolo Bettotti, Asiye Malkoç, Marco Canossa, and Lorenzo Pavesi "Set-ups to induce by light a memory in in-vitro neuronal cultures", Proc. SPIE 11947, Optogenetics and Optical Manipulation 2022, 1194703 (7 March 2022); https://doi.org/10.1117/12.2606876

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