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The laser treatment is an ideal option to conduct a surgical resection since it enables to provide higher accuracy and to
cause less damage when targeting invariant tissue areas. Compared to traditional mechanical or chemical treatment
modalities, it can shrink or destroy cancer cells using a narrow, focused light beam without harming normal tissue nearby.
Laser speckle contrast image (LSCI) is a powerful technology that is able to capture dynamic changes of backscattered
light, and then, convert it to relative flow using speckle contrast analysis. When the coherent light illuminates biological
tissue, random interference patterns will be received at the detector. If the particles are moving, patterns will result in
fluctuation as known as speckle patterns. By setting the exposure time equal to or longer than the speckle fluctuation
duration, such intensity fluctuation can be recorded, forming the speckle image. Since red blood cell is the primary
moving particles in vessels, the blurring can be related to flow information. Previous reports have also indicated that the
autocorrelation time is inversely related to the blood flow velocity. As a result, microvasculature and blood flow data can
be represented by analyzing the speckle variance of biological tissue spatially and temporarily. In this study, we propose
to use LSCI to in vivo investigate the induced thermal effect by laser illumination, and the difference in the thermal effect
caused by the CW and pulsed lasers will be compared. Finally, the treatment outcomes on brain tumor will be also
compared.
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