Multicolor tracking of single biomolecules with metallic nanoparticles at microsecond time resolution

Jun Ando

doi:10.1117/12.2615669

27 October 2021 Multicolor tracking of single biomolecules with metallic nanoparticles at microsecond time resolution

Jun Ando

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119250P (2021) https://doi.org/10.1117/12.2615669
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

Fast motions of biological molecules, such as lipids and proteins, have been observed with gold nanoparticles that work as contrast agent for optical microscopy. Because of the strong scattering signal of gold nanoparticles, motions of the biomolecules can be investigated with high temporal resolution and spatial precision. To further analyze complex interactions of multiple biological molecules, development of multicolor imaging system with metallic nanoparticles is important. Here, we developed a multicolor imaging system using silver and silver-gold alloy nanoparticles together with gold nanoparticles. We used multiple lasers that matched with plasmon resonance of each metal nanoparticles, and constructed a total internal reflection multicolor dark-field microscope with spectrophotometer. Fast and complex motions of multiple phospholipids in supported membranes, and multiple kinesins were observed at 100 μs time resolution.

Citation Download Citation

Jun Ando "Multicolor tracking of single biomolecules with metallic nanoparticles at microsecond time resolution", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119250P (27 October 2021); https://doi.org/10.1117/12.2615669

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