Deep ultraviolet based serial block-face imaging for three dimensional morphological assessment of the rodent brains

Deepa Kasaragod; Meina Zhu; Hidenori Aizawa

doi:10.1117/12.2615413

27 October 2021 Deep ultraviolet based serial block-face imaging for three dimensional morphological assessment of the rodent brains

Deepa Kasaragod, Meina Zhu, Hidenori Aizawa

Author Affiliations +

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 1192509 (2021) https://doi.org/10.1117/12.2615413
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

We present a deep ultraviolet (DUV) light microscope capable of serial block-face imaging of the whole rodent brain. The capability of the 3D-DUV microscope for quantitative volumetric analysis of the small substructures of habenula is shown. It is expected that large scale wide-field high resolution analysis of the smaller regions would allow for elucidating the functional changes linked with the morphological changes in brain under pathological conditions.

Citation Download Citation

Deepa Kasaragod, Meina Zhu, and Hidenori Aizawa "Deep ultraviolet based serial block-face imaging for three dimensional morphological assessment of the rodent brains", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 1192509 (27 October 2021); https://doi.org/10.1117/12.2615413

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