Noninvasive quantitative imaging of historical parchments by polarization-resolved SHG microscopy

Gaël Latour; Margaux Schmeltz; Laurianne Robinet; Sylvie Heu-Thao; Jean-Marc Sintès; Claire Teulon; Guillaume Ducourthial; Pierre Mahou; Marie-Claire Schanne-Klein

doi:10.1117/12.2593824

21 June 2021 Noninvasive quantitative imaging of historical parchments by polarization-resolved SHG microscopy

Gaël Latour, Margaux Schmeltz, Laurianne Robinet, Sylvie Heu-Thao, Jean-Marc Sintès, Claire Teulon, Guillaume Ducourthial, Pierre Mahou, Marie-Claire Schanne-Klein

Author Affiliations +

Proceedings Volume 11784, Optics for Arts, Architecture, and Archaeology VIII; 1178411 (2021) https://doi.org/10.1117/12.2593824
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

Advanced nonlinear optical (NLO) microscopy enables quantitative in situ mapping of parchment degradation at the micrometer scale. We show that measurements of the ratio of fluorescence over second harmonic generation (SHG) signals probes severe collagen degradation and could help to identify the parchments most at risk, close to the irreversible collagen denaturation into gelatin. Conversely, the anisotropy parameter obtained from polarization-resolved SHG measurements probes the earlier stages of degradation. This approach is first validated by comparing NLO quantitative parameters to thermal measurements by differential scanning calorimetry on artificially altered contemporary parchments. We then analyze invaluable parchments from the Middle Ages.

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Gaël Latour, Margaux Schmeltz, Laurianne Robinet, Sylvie Heu-Thao, Jean-Marc Sintès, Claire Teulon, Guillaume Ducourthial, Pierre Mahou, and Marie-Claire Schanne-Klein "Noninvasive quantitative imaging of historical parchments by polarization-resolved SHG microscopy", Proc. SPIE 11784, Optics for Arts, Architecture, and Archaeology VIII, 1178411 (21 June 2021); https://doi.org/10.1117/12.2593824

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