LED illumination for fluorescence from Fura-2 to Cy7.5 and beyond: A true lamp replacement

Kavita Aswani

doi:10.1117/12.2576651

5 March 2021 LED illumination for fluorescence from Fura-2 to Cy7.5 and beyond: A true lamp replacement

Kavita Aswani

Proceedings Volume 11706, Light-Emitting Devices, Materials, and Applications XXV; 117061K (2021) https://doi.org/10.1117/12.2576651
Event: SPIE OPTO, 2021, Online Only

Conference Poster

Abstract

Microscopy in the life sciences is moving from traditional Mercury and Xenon lamp to LED technology. LEDs provide long lifetimes, increased stability and no need for replacing or disposing of toxic bulbs. Applications that require uniform spectral stability across the near-UV to visible spectrum have relied on Xenon lamps. Fura-2 applications have relied on Xenon lamps for decades despite its low optical power. We present a new LED illumination system that can cover applications from Fura-2 to Cy7.5. A single device that can excite common fluorophores between 340nm and 800nm, with no compromise while the user benefits from LED technology.

Citation Download Citation

Kavita Aswani "LED illumination for fluorescence from Fura-2 to Cy7.5 and beyond: A true lamp replacement", Proc. SPIE 11706, Light-Emitting Devices, Materials, and Applications XXV, 117061K (5 March 2021); https://doi.org/10.1117/12.2576651

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