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We demonstrate the microfabrication of double-network (DN) hydrogels by multi-photon polymerization induced by focused femtosecond laser pulses. Two different poly (ethylene glycol) diacrylate (PEGDA) solutions (Mw=700 and 4000) were cured as both structures were crossing each other, allowing overlapped structure to form double networks composed of both employed polymer chains. This method allows the spatially-selective microfabrication of DN hydrogels inside the microfabricated hydrogel structures, which would realize the hydrogel microstructures showing spatially varied distribution of strength and stiffness.
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