Deep-UV hyperspectral microscopy with phase and absorption-based contrast

Francisco E. Robles

doi:10.1117/12.2582174

5 March 2021 Deep-UV hyperspectral microscopy with phase and absorption-based contrast

Francisco E. Robles

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116530F (2021) https://doi.org/10.1117/12.2582174
Event: SPIE BiOS, 2021, Online Only

Abstract

Ultraviolet hyperspectral interferometric (UHI) microscopy is a novel molecular imaging technique that retrieves wideband, high-resolution spectral information of the wavelength-dependent real part of the refractive index (i.e., dispersion), and the total attenuation coefficient of biological samples in the deep-ultraviolet (UV) spectral region. Here I will discuss the design of the system and processing methods used to extract the hyperspectral information. I will also present results from individual cells, tissues, and a detail study of spectral properties of several important biomolecules. Finally, biomedical applications and future directions will be discussed.

Conference Presentation

Citation Download Citation

Francisco E. Robles "Deep-UV hyperspectral microscopy with phase and absorption-based contrast", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116530F (5 March 2021); https://doi.org/10.1117/12.2582174

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