Metabolic imaging and secondary ion mass spectrometry analysis to investigate liver structure and function during regeneration

Daria S. Kuznetsova; Svetlana A. Rodimova; Nikolai V. Bobrov M.D.; Alexander A. Gulin; Vladislav I. Shcheslavskiy; Vladimir E. Zagainov; Elena V. Zagaynova M.D.

doi:10.1117/12.2584022

5 March 2021 Metabolic imaging and secondary ion mass spectrometry analysis to investigate liver structure and function during regeneration

Daria S. Kuznetsova, Svetlana A. Rodimova, Nikolai V. Bobrov M.D., Alexander A. Gulin, Vladislav I. Shcheslavskiy, Vladimir E. Zagainov, Elena V. Zagaynova M.D.

Author Affiliations +

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116481K (2021) https://doi.org/10.1117/12.2584022
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

We analyzed the structural and functional state of liver tissue in normal state and during regeneration using multiphoton microscopy in combination with the time-of-flight secondary ions mass spectrometry (TOF-SIMS). The induction of regeneration process was performed on 30% and 70% hepatectomy models of rats. The FLIM method has shown an increase in the contribution of the bound form of NADH, as well as the contribution of NADPH in hepatocytes, which indicates a growth in the contribution of oxidative phosphorylation and biosynetic processes during liver regeneration. Using the TOF-SIMS method, it has been shown that the signal from fragments of fats, fatty acids, phosphotidylcholine, and sphingomyelin is increased, combined with a decline in the signal from amino acid’s fragments in the regenerating liver. The results will make it possible to identify criteria for assessing the regenerative potential of the liver during resection in order to reduce the risk of developing of the postoperative liver failure.

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Daria S. Kuznetsova, Svetlana A. Rodimova, Nikolai V. Bobrov M.D., Alexander A. Gulin, Vladislav I. Shcheslavskiy, Vladimir E. Zagainov, and Elena V. Zagaynova M.D. "Metabolic imaging and secondary ion mass spectrometry analysis to investigate liver structure and function during regeneration", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116481K (5 March 2021); https://doi.org/10.1117/12.2584022

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